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anti cd4  (Bio X Cell)


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    Structured Review

    Bio X Cell anti cd4
    Anti Cd4, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4/InVivoMAb+anti-mouse+CD4/pm42864360-447-3-11
    Average 97 stars, based on 775 article reviews
    anti cd4 - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Lineage tracing of both quiescent G0 and active Hoxb5+ LT-HSCs that actively contribute to homeostatic mouse hematopoiesis
    Article Snippet: .. The spleen was stained with antibodies against Nk1.1 (BioLegend, 108706), CD4 (Bio X Cell, BE0003-1), Ly6C (BioLegend, 128012), CD8 (BD, 612759), Ter119 (BioLegend, 116220), CD19 (BD 563333), CD11b (BD, 563168), CD3 (BioLegend, 100237), CD45.2 (BD, 562895), and Ly6G (BD, 563978). .. The thymus was stained with antibodies against CD4 (Bio X Cell, BE0003-1), CD8 (BD, 612759), CD3 (BioLegend, 100237), Ter119 (BioLegend, 116220), and CD45.2 (BD, 562895).

    Article Title: Lineage tracing of both quiescent G0 and active Hoxb5+ LT-HSCs that actively contribute to homeostatic mouse hematopoiesis.
    Article Snippet: The cells were treated with ACK Lysis buffer (Invitrogen). .. Peripheral blood cells were then stained with antibodies against Nk1.1 (BioLegend, 108706), CD4 (Bio X Cell, BE0003-1), Ly6C (BioLegend, 128012), CD8 (BD, 612759), Ter119 (BioLegend, 116220), CD19 (BD 563333), CD11b (BD, 563168), CD3 (BioLegend, 100237), CD45.2 (BD, 562895), and Ly6G (BD, 563978). ..

    Article Title: Lineage tracing of both quiescent G0 and active Hoxb5+ LT-HSCs that actively contribute to homeostatic mouse hematopoiesis.
    Article Snippet: .. The spleen was stained with antibodies against Nk1.1 (BioLegend, 108706), CD4 (Bio X Cell, BE0003-1), Ly6C (BioLegend, 128012), CD8 (BD, 612759), Ter119 (BioLegend, 116220), CD19 (BD 563333), CD11b (BD, 563168), CD3 (BioLegend, 100237), CD45.2 (BD, 562895), and Ly6G (BD, 563978). .. The thymus was stained with antibodies against CD4 (Bio X Cell, BE0003-1), CD8 (BD, 612759), CD3 (BioLegend, 100237), Ter119 (BioLegend, 116220), and CD45.2 (BD, 562895).

    Control:

    Article Title: PD-1 inhibitor improves radiosensitivity by tumor vessel normalization
    Article Snippet: .. For antibody depletion experiments, animals received 250 μg of depleting or isotype control monoclonal antibodies, including CD4 (clone YTS177, #BE0003-3, BioXCell), CD8 (clone 53-6.7, #BE0004-1, BioXCell), and rat IgG2a (clone 2A3, #BE0089, BioXCell) to deplete different T cell subsets. ..

    Article Title: PD-1 inhibitor improves radiosensitivity by tumor vessel normalization.
    Article Snippet: .. For antibody depletion experiments, animals received 250 μg of depleting or isotype control monoclonal antibodies, including CD4 (clone YTS177, #BE0003-3, BioXCell), CD8 (clone 53-6.7, #BE0004-1, BioXCell), and rat IgG2a (clone 2A3, #BE0089, BioXCell) to deplete different T cell subsets. ..

    Article Title: Interleukin 6 drives durable T cell-mediated immunity to pancreatic cancer.
    Article Snippet: .. 407 Antibody-based depletion 408 Mice were dosed intraperitoneally (IP) with either a combination of CD4 (BioXcell, #BE0003-1) 409 and CD8a (BioXcell, #BE0061) depletion antibodies, or Rat IgG2b isotype control antibody 410 (BioXcell, #BE0090), which were resuspended in InVivoPure ph7.0 Dilution Buffer (BioXcell, 411 #IP0070) per the manufacturer’s instructions. ..

    Article Title: ERBB2 signaling drives immune cell evasion and resistance against immunotherapy in small cell lung cancer.
    Article Snippet: .. Mice were randomly assigned to either control or immune cell depletion group, which received CD4 (clone GK1.5, BioXCell, Cat. #BE0003-1) and CD8a (clone 2.43, BioXCell, Cat. #BE0061) depleting antibodies (200 μg/mouse, Nature Communications | (2025) 16:10983 13 diluted in PBS) twice per week with beginning two days after subcutaneous tumor cell injection. ..

    Article Title: ERBB2 signaling drives immune cell evasion and resistance against immunotherapy in small cell lung cancer
    Article Snippet: .. Mice were randomly assigned to either control or immune cell depletion group, which received CD4 (clone GK1.5, BioXCell, Cat. #BE0003-1) and CD8a (clone 2.43, BioXCell, Cat. #BE0061) depleting antibodies (200 μg/mouse, diluted in PBS) twice per week with beginning two days after subcutaneous tumor cell injection. ..

    Bioprocessing:

    Article Title: PD-1 inhibitor improves radiosensitivity by tumor vessel normalization
    Article Snippet: .. For antibody depletion experiments, animals received 250 μg of depleting or isotype control monoclonal antibodies, including CD4 (clone YTS177, #BE0003-3, BioXCell), CD8 (clone 53-6.7, #BE0004-1, BioXCell), and rat IgG2a (clone 2A3, #BE0089, BioXCell) to deplete different T cell subsets. ..

    Article Title: PD-1 inhibitor improves radiosensitivity by tumor vessel normalization.
    Article Snippet: .. For antibody depletion experiments, animals received 250 μg of depleting or isotype control monoclonal antibodies, including CD4 (clone YTS177, #BE0003-3, BioXCell), CD8 (clone 53-6.7, #BE0004-1, BioXCell), and rat IgG2a (clone 2A3, #BE0089, BioXCell) to deplete different T cell subsets. ..

    Mouse Assay:

    Article Title: Interleukin 6 drives durable T cell-mediated immunity to pancreatic cancer.
    Article Snippet: .. 407 Antibody-based depletion 408 Mice were dosed intraperitoneally (IP) with either a combination of CD4 (BioXcell, #BE0003-1) 409 and CD8a (BioXcell, #BE0061) depletion antibodies, or Rat IgG2b isotype control antibody 410 (BioXcell, #BE0090), which were resuspended in InVivoPure ph7.0 Dilution Buffer (BioXcell, 411 #IP0070) per the manufacturer’s instructions. ..

    Article Title: ERBB2 signaling drives immune cell evasion and resistance against immunotherapy in small cell lung cancer.
    Article Snippet: .. Mice were randomly assigned to either control or immune cell depletion group, which received CD4 (clone GK1.5, BioXCell, Cat. #BE0003-1) and CD8a (clone 2.43, BioXCell, Cat. #BE0061) depleting antibodies (200 μg/mouse, Nature Communications | (2025) 16:10983 13 diluted in PBS) twice per week with beginning two days after subcutaneous tumor cell injection. ..

    Article Title: ERBB2 signaling drives immune cell evasion and resistance against immunotherapy in small cell lung cancer
    Article Snippet: .. Mice were randomly assigned to either control or immune cell depletion group, which received CD4 (clone GK1.5, BioXCell, Cat. #BE0003-1) and CD8a (clone 2.43, BioXCell, Cat. #BE0061) depleting antibodies (200 μg/mouse, diluted in PBS) twice per week with beginning two days after subcutaneous tumor cell injection. ..

    Injection:

    Article Title: ERBB2 signaling drives immune cell evasion and resistance against immunotherapy in small cell lung cancer.
    Article Snippet: .. Mice were randomly assigned to either control or immune cell depletion group, which received CD4 (clone GK1.5, BioXCell, Cat. #BE0003-1) and CD8a (clone 2.43, BioXCell, Cat. #BE0061) depleting antibodies (200 μg/mouse, Nature Communications | (2025) 16:10983 13 diluted in PBS) twice per week with beginning two days after subcutaneous tumor cell injection. ..

    Article Title: ERBB2 signaling drives immune cell evasion and resistance against immunotherapy in small cell lung cancer
    Article Snippet: .. Mice were randomly assigned to either control or immune cell depletion group, which received CD4 (clone GK1.5, BioXCell, Cat. #BE0003-1) and CD8a (clone 2.43, BioXCell, Cat. #BE0061) depleting antibodies (200 μg/mouse, diluted in PBS) twice per week with beginning two days after subcutaneous tumor cell injection. ..



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    Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized <t>lymphocytes,</t> with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.
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    Image Search Results


    Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized lymphocytes, with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.

    Journal: Veterinary Medicine and Science

    Article Title: Rare Feline Gingival Extramedullary Plasmacytoma: Clinical Application of Electrochemotherapy for Local Control

    doi: 10.1002/vms3.71294

    Figure Lengend Snippet: Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized lymphocytes, with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.

    Article Snippet: For flow cytometric analysis, antibodies against CD21 (B‐cell marker; clone CA2.1D6, RPE‐conjugated, Bio‐Rad Antibodies, MCA1781R), CD5 (pan–T‐cell marker; clone FE1.1B11, FITC‐conjugated, Bio‐Rad Antibodies, MCA2038F), CD4 (helper T‐cell marker; clone VPG34, FITC‐conjugated; Bio‐Rad Antibodies, MCA1346F) and CD8 (cytotoxic T‐cell marker; clone VPG9, RPE‐conjugated; Bio‐Rad Antibodies, MCA1347R) were used.

    Techniques: Immunohistochemical staining, Staining, Expressing

    (A) Representative dot plots of isolated peripheral blood mononuclear cells (PBMCs) before (upper row) and after (lower row) total CD4 + cells magnetic‐activated cell sorting (MACS) from a young donor. Cells are gated on the living population and checked for CD4 vs. CD8 (left panels), CD45RA vs. CD28 expression (middle panels), and for CD127 vs. CD25 (right panels) expression. Frequencies of CD4 + Foxp3 + (B) and CD25 + of CD4 + Foxp3 + (C) cells are shown after 4 days of in vitro induction of Treg cells in the presence of 10% fetal calf serum (FCS) containing medium RPMI or in serum‐free medium XVivo‐15 (MEAN; n = 2, young donors).

    Journal: FEBS Open Bio

    Article Title: In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

    doi: 10.1002/2211-5463.70349

    Figure Lengend Snippet: (A) Representative dot plots of isolated peripheral blood mononuclear cells (PBMCs) before (upper row) and after (lower row) total CD4 + cells magnetic‐activated cell sorting (MACS) from a young donor. Cells are gated on the living population and checked for CD4 vs. CD8 (left panels), CD45RA vs. CD28 expression (middle panels), and for CD127 vs. CD25 (right panels) expression. Frequencies of CD4 + Foxp3 + (B) and CD25 + of CD4 + Foxp3 + (C) cells are shown after 4 days of in vitro induction of Treg cells in the presence of 10% fetal calf serum (FCS) containing medium RPMI or in serum‐free medium XVivo‐15 (MEAN; n = 2, young donors).

    Article Snippet: For the purification of total CD4 + T cells, we used the Miltenyi Biotec CD4 T‐cell Kit (130‐096‐533) according to the manufacturer's protocol.

    Techniques: Isolation, FACS, Expressing, In Vitro

    Frequency of Foxp3 + CD4 + cells (A) and CD25 expression in Foxp3 + cells (B) after 6 days of induction in TCR‐stimulated cells in the presence of IL‐2 (iTreg IL‐2 ) only or IL‐2 + TGF‐β (iTreg TGFβ ) in young and older individuals. (Mean ± SEM; n ≥ 3). Statistical significance P < 0.05*, and P < 0.01** (two‐way ANOVA with Sidak's post hoc test).

    Journal: FEBS Open Bio

    Article Title: In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

    doi: 10.1002/2211-5463.70349

    Figure Lengend Snippet: Frequency of Foxp3 + CD4 + cells (A) and CD25 expression in Foxp3 + cells (B) after 6 days of induction in TCR‐stimulated cells in the presence of IL‐2 (iTreg IL‐2 ) only or IL‐2 + TGF‐β (iTreg TGFβ ) in young and older individuals. (Mean ± SEM; n ≥ 3). Statistical significance P < 0.05*, and P < 0.01** (two‐way ANOVA with Sidak's post hoc test).

    Article Snippet: For the purification of total CD4 + T cells, we used the Miltenyi Biotec CD4 T‐cell Kit (130‐096‐533) according to the manufacturer's protocol.

    Techniques: Expressing